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anti p plcγ 1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p plcγ 1
    Anti P Plcγ 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+plc%CE%B3/Phospho-PLCgamma1+(Tyr783)+Rabbit+mAb/bio_rxiv__64898__2026__03__19__712941-261-16-29
    Average 95 stars, based on 167 article reviews
    anti p plcγ 1 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
    Article Snippet: Antibody , Anti-PLCγ (rabbit polyclonal) , Cell Signaling , 2822S , (1:1000).

    Labeling:

    Article Title: Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters.
    Article Snippet: .. The antibodies and reagents were purchased as follows: anti-IL-2 (1:500, JES6-1A12, e-Bioscience, 14-7022-85, RRID:AB_468406), biotinlabeled anti-IL-2 (1:1000, JES6-5H4, e-Bioscience, 13-7021-85, RRID:AB_466899), PE–anti-I-A/I-E (2.5μg/ml, M5/114.15.2, BioLegend, 107608, RRID:AB_313323), PE–anti-mPD-1 (2.5 μg/ml, 29 F.1A12, BioLegend, 135206, RRID:AB_1877231), anti-mPD-1 (2.5μg/ml, 29F.1A12, BioLegend, 135248, RRID:AB_2783091), anti-hPD-L1 (2.5μg/ml, 29E.2A3, BioLegend, 329746, RRID:AB_2783199), and anti-hPD-L2 (2.5μg/ml, 24F.10C12, BioLegend, 329624, RRID:AB_2819957), PE–anti-hPD-1 (10μl, MIH4, BD bioscience, 557946, RRID:AB_647199), PE–anti-hPD-L1 (10μl, MIH1, BD bioscience, 557924, RRID:AB_647198), PE–anti-hPD-L2 (10μl, MIH18, BD bioscience, 558066, RRID: AB_647197), Alexa Fluor 647–labeled anti-pCD3ζ (2.5μg/ml, K25407.69, BD bioscience, 558489, RRID: AB_647152), and Alexa Fluor 647–labeled anti-pSLP-76 (2.5μg/ml, J141-668.36.58, BD bioscience, 558438, RRID:AB_647159), rabbit polyclonal anti-SHP1 (1:500, C-19, Santa Cruz Biotechnology Inc., sc-287, RRID:AB_2173829) and mouse anti-SHP2 (1:1000, B-1, Santa Cruz Biotechnology Inc., sc-7384, RRID:AB_ 628252), anti-Erk (1:1000, Cell Signaling Technology, 4695S, RRID: AB_390779), anti-pErk (1:1000, Cell Signaling Technology, 4370S, RRID: AB_2315112), anti-PLCγ (1:1000, Cell Signaling Technology, 5690S, RRID: AB_10691383), anti-pPLCγ (1:1000, Cell Signaling Technology, 8713S, RRID: AB_10890863), anti-Akt (1:2000, Cell Signaling Technology, 4691S, RRID: AB_915783), anti-pAkt (1:1000, Cell Signaling Technology, 4060S, RRID: AB_2315049), and HRP-anti-rabbit IgG polyclonal Abs (1:10,000, Cell Signaling Technology, 7074S, RRID: AB_2099233), HRP-anti-mouse IgG polyclonal Abs (1:10,000, Cappel, 55550), pembrolizumab (MCE, HY-P9902), nivolumab (MCE, HYP9903), durvalumab (MCE, HY-P9919), and atezolizumab (MCE, HYP9904), APC-anti-human IgG (H+ L) (2.5μg/ml, Jackson Immuno Research, 705-136-147, RRID: AB_2340407), DyLight 650 and 549 labeling kits (Thermo Fisher Scientific, 84535 and 53044), HaloTag (HT) STELLA Fluor 650 and TMR ligands (Promega, GCKA308-01 and G8252), SNAP-Cell 647-Sir (New England BioLabs, S9102S), MCC88-103 (ANERADLIAYLKQATK, GenScript), and OVA257-264 (SIINFEKL, GenScript) peptides. .. A B cell hybridoma producing anti-CD28 (PV-1) was provided by R. Abe (Tokyo University of Science, Noda, Japan); antiCD3ζ (145-2c11) by J. Bluestone (University of California, San Francisco, USA); anti-TCRβ (H57-597) by R. T. Kubo (Cytel Corp., CA, USA); and anti-I-Ek (14-4-4) and anti-ICAM-1 (YN1/1.7.4) byM.

    Article Title: Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters
    Article Snippet: .. The antibodies and reagents were purchased as follows: anti-IL-2 (1:500, JES6-1A12, e-Bioscience, 14-7022-85, RRID:AB_468406), biotin-labeled anti-IL-2 (1:1000, JES6-5H4, e-Bioscience, 13-7021-85, RRID:AB_466899), PE–anti-I-A/I-E (2.5 μg/ml, M5/114.15.2, BioLegend, 107608, RRID:AB_313323), PE–anti-mPD-1 (2.5 μg/ml, 29 F.1A12, BioLegend, 135206, RRID:AB_1877231), anti-mPD-1 (2.5 μg/ml, 29F.1A12, BioLegend, 135248, RRID:AB_2783091), anti-hPD-L1 (2.5 μg/ml, 29E.2A3, BioLegend, 329746, RRID:AB_2783199), and anti-hPD-L2 (2.5 μg/ml, 24F.10C12, BioLegend, 329624, RRID:AB_2819957), PE–anti-hPD-1 (10 μl, MIH4, BD bioscience, 557946, RRID:AB_647199), PE–anti-hPD-L1 (10 μl, MIH1, BD bioscience, 557924, RRID:AB_647198), PE–anti-hPD-L2 (10 μl, MIH18, BD bioscience, 558066, RRID: AB_647197), Alexa Fluor 647–labeled anti-pCD3ζ (2.5 μg/ml, K25-407.69, BD bioscience, 558489, RRID: AB_647152), and Alexa Fluor 647–labeled anti-pSLP-76 (2.5 μg/ml, J141-668.36.58, BD bioscience, 558438, RRID:AB_647159), rabbit polyclonal anti-SHP1 (1:500, C-19, Santa Cruz Biotechnology Inc., sc-287, RRID:AB_2173829) and mouse anti-SHP2 (1:1000, B-1, Santa Cruz Biotechnology Inc., sc-7384, RRID:AB_ 628252), anti-Erk (1:1000, Cell Signaling Technology, 4695S, RRID: AB_390779), anti-pErk (1:1000, Cell Signaling Technology, 4370S, RRID: AB_2315112), anti-PLCγ (1:1000, Cell Signaling Technology, 5690S, RRID: AB_10691383), anti-pPLCγ (1:1000, Cell Signaling Technology, 8713S, RRID: AB_10890863), anti-Akt (1:2000, Cell Signaling Technology, 4691S, RRID: AB_915783), anti-pAkt (1:1000, Cell Signaling Technology, 4060S, RRID: AB_2315049), and HRP-anti-rabbit IgG polyclonal Abs (1:10,000, Cell Signaling Technology, 7074S, RRID: AB_2099233), HRP-anti-mouse IgG polyclonal Abs (1:10,000, Cappel, 55550), pembrolizumab (MCE, HY-P9902), nivolumab (MCE, HY-P9903), durvalumab (MCE, HY-P9919), and atezolizumab (MCE, HY-P9904), APC-anti-human IgG (H + L) (2.5 μg/ml, Jackson Immuno Research, 705-136-147, RRID: AB_2340407), DyLight 650 and 549 labeling kits (Thermo Fisher Scientific, 84535 and 53044), HaloTag (HT) STELLA Fluor 650 and TMR ligands (Promega, GCKA308-01 and G8252), SNAP-Cell 647-Sir (New England BioLabs, S9102S), MCC 88-103 (ANERADLIAYLKQATK, GenScript), and OVA 257-264 (SIINFEKL, GenScript) peptides. .. A B cell hybridoma producing anti-CD28 (PV-1) was provided by R. Abe (Tokyo University of Science, Noda, Japan); anti-CD3ζ (145-2c11) by J. Bluestone (University of California, San Francisco, USA); anti-TCRβ (H57-597) by R. T. Kubo (Cytel Corp., CA, USA); and anti-I-E k (14-4-4) and anti-ICAM-1 (YN1/1.7.4) by M. L. Dustin (University of Oxford, UK); anti-hPD-L1 (MIH1) and anti-hPD-L2 (MIH18) , by M. Azuma (Tokyo Medical and Dental University, Tokyo, Japan).

    Immunoprecipitation:

    Article Title: Composition and method for modulating fibroblast growth factor receptor 3 activation
    Article Snippet: For experiments not requiring immunoprecipitation, lysates were immunoblotted with anti-FGFR3 (C-15), anti-RrGFP (1-233), anti-pFGFR3 (3471; Cell Signaling, Beverly, Mass., USA), anti-PLCγ (2822; Cell Signaling), anti-pPLCγ (2821; Cell Signaling), anti-ERK1 C-16 (SC-123; Santa Cruz Biotechnology), anti-ERK1/2 Thr220/Thr204 (9101; Cell Signaling), anti-PI3K p85 (#4292, Cell Signaling), anti-pPI3K p85 alpha Tyr 508 (Sc-123, Santa Cruz), anti-STATS 3H7 (#9358, Cell Signaling), anti-pSTAT5 Tyr694 (#9359, Cell Signaling) or anti-β-actin (MBA1501; Millipore) antibodies, followed by detection using enhanced chemiluminescence (ECL; Amersham Biosciences, Little Chalfont, Buckinghamshire, UK). .. For experiments not requiring immunoprecipitation, lysates were immunoblotted with anti-FGFR3 (C-15), anti-RrGFP (1-233), anti-pFGFR3 (3471; Cell Signaling, Beverly, Mass., USA), anti-PLCγ (2822; Cell Signaling), anti-pPLCγ (2821; Cell Signaling), anti-ERK1 C-16 (SC-123; Santa Cruz Biotechnology), anti-ERK1/2 Thr220/Thr204 (9101; Cell Signaling), anti-PI3K p85 (#4292, Cell Signaling), anti-pPI3K p85 alpha Tyr 508 (Sc-123, Santa Cruz), anti-STATS 3H7 (#9358, Cell Signaling), anti-pSTAT5 Tyr694 (#9359, Cell Signaling) or anti-β-actin (MBA1501; Millipore) antibodies, followed by detection using enhanced chemiluminescence (ECL; Amersham Biosciences, Little Chalfont, Buckinghamshire, UK). .. The culture medium was replaced with serum-free medium for assay of kinase inhibition by PKC412, and live cells were imaged on an UltraView inverted confocal microscope (Perkin Elmer, Mass., USA).

    Article Title: Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters.
    Article Snippet: .. Whole cell lysates (WCLs) or those immunoprecipitated by anti-GFP (MBL International, D153-11, RRID:AB_2893312) were blotted with antiGFP (1:5000, Miltenyi Biotec, 130-091-833, RRID:AB_247003), antimSHP1 (1:500, Santa Cruz Biotechnology, sc-287, RRID:AB_2173829), anti-mSHP2 (1:1000, Santa Cruz Biotechnology Inc., sc-7384, RRID:AB_628252), anti-PLCγ (1:1000, Cell Signaling Technology, 5690, RRID:AB_10691383), anti-pPLCγ (1:1000, Cell Signaling Technology, 8713, RRID:AB_10890863), anti-Akt (1:2000, Cell Signaling Technology, 4691, RRID:AB_915783), anti-pAkt (1:1000, Cell Signaling Technology, 4060, RRID:AB_2315049), anti-Erk (1:1000, Cell Signaling Technology, 4695, RRID:AB_390779), or anti-pErk (1:1000, Cell Signaling Technology, 4370, RRID:AB_2315112) as a first antibody andHRP-anti-rabbit IgG polyclonal Abs (1:10,000, Cell Signaling Technology, 7074, RRID:AB_2099233) as a second one. .. Each intensity of band was calculated by ImageJ (RRID:SCR_003070).

    Article Title: Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters
    Article Snippet: .. Whole cell lysates (WCLs) or those immunoprecipitated by anti-GFP (MBL International, D153-11, RRID:AB_2893312) were blotted with anti-GFP (1:5000, Miltenyi Biotec, 130-091-833, RRID:AB_247003), anti-mSHP1 (1:500, Santa Cruz Biotechnology, sc-287, RRID:AB_2173829), anti-mSHP2 (1:1000, Santa Cruz Biotechnology Inc., sc-7384, RRID:AB_628252), anti-PLCγ (1:1000, Cell Signaling Technology, 5690, RRID:AB_10691383), anti-pPLCγ (1:1000, Cell Signaling Technology, 8713, RRID:AB_10890863), anti-Akt (1:2000, Cell Signaling Technology, 4691, RRID:AB_915783), anti-pAkt (1:1000, Cell Signaling Technology, 4060, RRID:AB_2315049), anti-Erk (1:1000, Cell Signaling Technology, 4695, RRID:AB_390779), or anti-pErk (1:1000, Cell Signaling Technology, 4370, RRID:AB_2315112) as a first antibody and HRP-anti-rabbit IgG polyclonal Abs (1:10,000, Cell Signaling Technology, 7074, RRID:AB_2099233) as a second one. .. Each intensity of band was calculated by ImageJ (RRID:SCR_003070).

    Phospho-proteomics:

    Article Title: Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectin.
    Article Snippet: Cells were spread on glass slides, fixed with paraformaldehyde for 20 min, and observed under a laser confocal microscope (LSM 700, ZEISS) after adding DAPI. .. Coimmunoprecipitation, phosphorylation assay, and Western blot For coimmunoprecipitation (co- IP) and Western blot, we used anti- TCRɑ (sc- 515719, Santa Cruz Biotechnology), anti- TCRβ (9485, Cell Signaling Technology), anti- CD3ζ (A11157, ABclonal), anti- CD3γ (A4085, ABclonal), anti- CD3δ (A9770, ABclonal), anti- CD3ε (sc- 20047, Santa Cruz Biotechnology), anti- pCD3ζ-Y142 (ab68235,Abcam), anti- LCK (2657, Cell Signaling Technology), anti- pLCK- Y394 (AP0182, ABclonal), anti- PLCγ (2822, Cell Signaling Technology), antipPLCγ (2821, Cell Signaling Technology), anti- NFATc1 (5861, Cell Signaling Technology), anti- NF-κB p65 (6956, Cell Signaling Technology), anti- ZAP70 (3165, Cell Signaling Technology), anti- pZap70 (2701, Cell Signaling Technology), peroxidase- conjugated goat anti- rabbit IgG (33 101ES60, Yeasen), peroxidase AffiniPure goat anti- mouse IgG (33 201ES60, Yeasen), streptavidin magnetic beads (P2151, Beyotime Biotechnology), NP- 40 lysis buffer (P0013F, Beyotime Biotechnology), Nuclear and Cytoplasmic Protein Extraction Kit (20 126ES50, Yeasen), protease inhibitor cocktail (20 124ES03, Yeasen), phosphatase inhibitor cocktail (20 109ES05,Yeasen), and a co- IP kit (26149, Thermo Fisher Scientific). ..

    Western Blot:

    Article Title: Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectin.
    Article Snippet: Cells were spread on glass slides, fixed with paraformaldehyde for 20 min, and observed under a laser confocal microscope (LSM 700, ZEISS) after adding DAPI. .. Coimmunoprecipitation, phosphorylation assay, and Western blot For coimmunoprecipitation (co- IP) and Western blot, we used anti- TCRɑ (sc- 515719, Santa Cruz Biotechnology), anti- TCRβ (9485, Cell Signaling Technology), anti- CD3ζ (A11157, ABclonal), anti- CD3γ (A4085, ABclonal), anti- CD3δ (A9770, ABclonal), anti- CD3ε (sc- 20047, Santa Cruz Biotechnology), anti- pCD3ζ-Y142 (ab68235,Abcam), anti- LCK (2657, Cell Signaling Technology), anti- pLCK- Y394 (AP0182, ABclonal), anti- PLCγ (2822, Cell Signaling Technology), antipPLCγ (2821, Cell Signaling Technology), anti- NFATc1 (5861, Cell Signaling Technology), anti- NF-κB p65 (6956, Cell Signaling Technology), anti- ZAP70 (3165, Cell Signaling Technology), anti- pZap70 (2701, Cell Signaling Technology), peroxidase- conjugated goat anti- rabbit IgG (33 101ES60, Yeasen), peroxidase AffiniPure goat anti- mouse IgG (33 201ES60, Yeasen), streptavidin magnetic beads (P2151, Beyotime Biotechnology), NP- 40 lysis buffer (P0013F, Beyotime Biotechnology), Nuclear and Cytoplasmic Protein Extraction Kit (20 126ES50, Yeasen), protease inhibitor cocktail (20 124ES03, Yeasen), phosphatase inhibitor cocktail (20 109ES05,Yeasen), and a co- IP kit (26149, Thermo Fisher Scientific). ..

    Co-Immunoprecipitation Assay:

    Article Title: Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectin.
    Article Snippet: Cells were spread on glass slides, fixed with paraformaldehyde for 20 min, and observed under a laser confocal microscope (LSM 700, ZEISS) after adding DAPI. .. Coimmunoprecipitation, phosphorylation assay, and Western blot For coimmunoprecipitation (co- IP) and Western blot, we used anti- TCRɑ (sc- 515719, Santa Cruz Biotechnology), anti- TCRβ (9485, Cell Signaling Technology), anti- CD3ζ (A11157, ABclonal), anti- CD3γ (A4085, ABclonal), anti- CD3δ (A9770, ABclonal), anti- CD3ε (sc- 20047, Santa Cruz Biotechnology), anti- pCD3ζ-Y142 (ab68235,Abcam), anti- LCK (2657, Cell Signaling Technology), anti- pLCK- Y394 (AP0182, ABclonal), anti- PLCγ (2822, Cell Signaling Technology), antipPLCγ (2821, Cell Signaling Technology), anti- NFATc1 (5861, Cell Signaling Technology), anti- NF-κB p65 (6956, Cell Signaling Technology), anti- ZAP70 (3165, Cell Signaling Technology), anti- pZap70 (2701, Cell Signaling Technology), peroxidase- conjugated goat anti- rabbit IgG (33 101ES60, Yeasen), peroxidase AffiniPure goat anti- mouse IgG (33 201ES60, Yeasen), streptavidin magnetic beads (P2151, Beyotime Biotechnology), NP- 40 lysis buffer (P0013F, Beyotime Biotechnology), Nuclear and Cytoplasmic Protein Extraction Kit (20 126ES50, Yeasen), protease inhibitor cocktail (20 124ES03, Yeasen), phosphatase inhibitor cocktail (20 109ES05,Yeasen), and a co- IP kit (26149, Thermo Fisher Scientific). ..

    Magnetic Beads:

    Article Title: Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectin.
    Article Snippet: Cells were spread on glass slides, fixed with paraformaldehyde for 20 min, and observed under a laser confocal microscope (LSM 700, ZEISS) after adding DAPI. .. Coimmunoprecipitation, phosphorylation assay, and Western blot For coimmunoprecipitation (co- IP) and Western blot, we used anti- TCRɑ (sc- 515719, Santa Cruz Biotechnology), anti- TCRβ (9485, Cell Signaling Technology), anti- CD3ζ (A11157, ABclonal), anti- CD3γ (A4085, ABclonal), anti- CD3δ (A9770, ABclonal), anti- CD3ε (sc- 20047, Santa Cruz Biotechnology), anti- pCD3ζ-Y142 (ab68235,Abcam), anti- LCK (2657, Cell Signaling Technology), anti- pLCK- Y394 (AP0182, ABclonal), anti- PLCγ (2822, Cell Signaling Technology), antipPLCγ (2821, Cell Signaling Technology), anti- NFATc1 (5861, Cell Signaling Technology), anti- NF-κB p65 (6956, Cell Signaling Technology), anti- ZAP70 (3165, Cell Signaling Technology), anti- pZap70 (2701, Cell Signaling Technology), peroxidase- conjugated goat anti- rabbit IgG (33 101ES60, Yeasen), peroxidase AffiniPure goat anti- mouse IgG (33 201ES60, Yeasen), streptavidin magnetic beads (P2151, Beyotime Biotechnology), NP- 40 lysis buffer (P0013F, Beyotime Biotechnology), Nuclear and Cytoplasmic Protein Extraction Kit (20 126ES50, Yeasen), protease inhibitor cocktail (20 124ES03, Yeasen), phosphatase inhibitor cocktail (20 109ES05,Yeasen), and a co- IP kit (26149, Thermo Fisher Scientific). ..

    Lysis:

    Article Title: Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectin.
    Article Snippet: Cells were spread on glass slides, fixed with paraformaldehyde for 20 min, and observed under a laser confocal microscope (LSM 700, ZEISS) after adding DAPI. .. Coimmunoprecipitation, phosphorylation assay, and Western blot For coimmunoprecipitation (co- IP) and Western blot, we used anti- TCRɑ (sc- 515719, Santa Cruz Biotechnology), anti- TCRβ (9485, Cell Signaling Technology), anti- CD3ζ (A11157, ABclonal), anti- CD3γ (A4085, ABclonal), anti- CD3δ (A9770, ABclonal), anti- CD3ε (sc- 20047, Santa Cruz Biotechnology), anti- pCD3ζ-Y142 (ab68235,Abcam), anti- LCK (2657, Cell Signaling Technology), anti- pLCK- Y394 (AP0182, ABclonal), anti- PLCγ (2822, Cell Signaling Technology), antipPLCγ (2821, Cell Signaling Technology), anti- NFATc1 (5861, Cell Signaling Technology), anti- NF-κB p65 (6956, Cell Signaling Technology), anti- ZAP70 (3165, Cell Signaling Technology), anti- pZap70 (2701, Cell Signaling Technology), peroxidase- conjugated goat anti- rabbit IgG (33 101ES60, Yeasen), peroxidase AffiniPure goat anti- mouse IgG (33 201ES60, Yeasen), streptavidin magnetic beads (P2151, Beyotime Biotechnology), NP- 40 lysis buffer (P0013F, Beyotime Biotechnology), Nuclear and Cytoplasmic Protein Extraction Kit (20 126ES50, Yeasen), protease inhibitor cocktail (20 124ES03, Yeasen), phosphatase inhibitor cocktail (20 109ES05,Yeasen), and a co- IP kit (26149, Thermo Fisher Scientific). ..

    Protein Extraction:

    Article Title: Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectin.
    Article Snippet: Cells were spread on glass slides, fixed with paraformaldehyde for 20 min, and observed under a laser confocal microscope (LSM 700, ZEISS) after adding DAPI. .. Coimmunoprecipitation, phosphorylation assay, and Western blot For coimmunoprecipitation (co- IP) and Western blot, we used anti- TCRɑ (sc- 515719, Santa Cruz Biotechnology), anti- TCRβ (9485, Cell Signaling Technology), anti- CD3ζ (A11157, ABclonal), anti- CD3γ (A4085, ABclonal), anti- CD3δ (A9770, ABclonal), anti- CD3ε (sc- 20047, Santa Cruz Biotechnology), anti- pCD3ζ-Y142 (ab68235,Abcam), anti- LCK (2657, Cell Signaling Technology), anti- pLCK- Y394 (AP0182, ABclonal), anti- PLCγ (2822, Cell Signaling Technology), antipPLCγ (2821, Cell Signaling Technology), anti- NFATc1 (5861, Cell Signaling Technology), anti- NF-κB p65 (6956, Cell Signaling Technology), anti- ZAP70 (3165, Cell Signaling Technology), anti- pZap70 (2701, Cell Signaling Technology), peroxidase- conjugated goat anti- rabbit IgG (33 101ES60, Yeasen), peroxidase AffiniPure goat anti- mouse IgG (33 201ES60, Yeasen), streptavidin magnetic beads (P2151, Beyotime Biotechnology), NP- 40 lysis buffer (P0013F, Beyotime Biotechnology), Nuclear and Cytoplasmic Protein Extraction Kit (20 126ES50, Yeasen), protease inhibitor cocktail (20 124ES03, Yeasen), phosphatase inhibitor cocktail (20 109ES05,Yeasen), and a co- IP kit (26149, Thermo Fisher Scientific). ..

    Protease Inhibitor:

    Article Title: Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectin.
    Article Snippet: Cells were spread on glass slides, fixed with paraformaldehyde for 20 min, and observed under a laser confocal microscope (LSM 700, ZEISS) after adding DAPI. .. Coimmunoprecipitation, phosphorylation assay, and Western blot For coimmunoprecipitation (co- IP) and Western blot, we used anti- TCRɑ (sc- 515719, Santa Cruz Biotechnology), anti- TCRβ (9485, Cell Signaling Technology), anti- CD3ζ (A11157, ABclonal), anti- CD3γ (A4085, ABclonal), anti- CD3δ (A9770, ABclonal), anti- CD3ε (sc- 20047, Santa Cruz Biotechnology), anti- pCD3ζ-Y142 (ab68235,Abcam), anti- LCK (2657, Cell Signaling Technology), anti- pLCK- Y394 (AP0182, ABclonal), anti- PLCγ (2822, Cell Signaling Technology), antipPLCγ (2821, Cell Signaling Technology), anti- NFATc1 (5861, Cell Signaling Technology), anti- NF-κB p65 (6956, Cell Signaling Technology), anti- ZAP70 (3165, Cell Signaling Technology), anti- pZap70 (2701, Cell Signaling Technology), peroxidase- conjugated goat anti- rabbit IgG (33 101ES60, Yeasen), peroxidase AffiniPure goat anti- mouse IgG (33 201ES60, Yeasen), streptavidin magnetic beads (P2151, Beyotime Biotechnology), NP- 40 lysis buffer (P0013F, Beyotime Biotechnology), Nuclear and Cytoplasmic Protein Extraction Kit (20 126ES50, Yeasen), protease inhibitor cocktail (20 124ES03, Yeasen), phosphatase inhibitor cocktail (20 109ES05,Yeasen), and a co- IP kit (26149, Thermo Fisher Scientific). ..



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    <t>PLCγ/PKC</t> Pathway Protein and Gene Expression. (A) Immunoblot of PLCγ, p-PLCγ, PKC, p-PKC Proteins Expression in Testicular Tissues of Mice in Each Group; (B–E) Average Optical Densities of PLCγ, p-PLCγ, PKC and p-PKC Proteins Compared With That of β-Actin Protein, Respectively; (F&G) The mRNA Levels of PLCγ and PKC * p < .05 versus control group. # p < .05 versus model group. Data represent mean ± SD , n = 3. && p < .01 versus WZYZW low-dose group. $$ p < .01 versus WZYZW medium-dose group.
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    Image Search Results


    PLCγ/PKC Pathway Protein and Gene Expression. (A) Immunoblot of PLCγ, p-PLCγ, PKC, p-PKC Proteins Expression in Testicular Tissues of Mice in Each Group; (B–E) Average Optical Densities of PLCγ, p-PLCγ, PKC and p-PKC Proteins Compared With That of β-Actin Protein, Respectively; (F&G) The mRNA Levels of PLCγ and PKC * p < .05 versus control group. # p < .05 versus model group. Data represent mean ± SD , n = 3. && p < .01 versus WZYZW low-dose group. $$ p < .01 versus WZYZW medium-dose group.

    Journal: American Journal of Men's Health

    Article Title: Wu Zi Yan Zong Wan Improves Triptolide-Induced Testicular Spermatogenic Dysfunction Through the Spermatogenic Microenvironment and the PLCγ/PKC Pathway

    doi: 10.1177/15579883251371990

    Figure Lengend Snippet: PLCγ/PKC Pathway Protein and Gene Expression. (A) Immunoblot of PLCγ, p-PLCγ, PKC, p-PKC Proteins Expression in Testicular Tissues of Mice in Each Group; (B–E) Average Optical Densities of PLCγ, p-PLCγ, PKC and p-PKC Proteins Compared With That of β-Actin Protein, Respectively; (F&G) The mRNA Levels of PLCγ and PKC * p < .05 versus control group. # p < .05 versus model group. Data represent mean ± SD , n = 3. && p < .01 versus WZYZW low-dose group. $$ p < .01 versus WZYZW medium-dose group.

    Article Snippet: PLCγ (1:2000; Cell Signaling Technology Cat. No. 5385, RRID: AB_2797610, Rabbit monoclonal antibody, Boston, MA, USA), p-PLCγ (1:2000; Cell Signaling Technology Cat. No. 2821, RRID: AB_2798485, Rabbit monoclonal antibody, Boston, MA, USA).

    Techniques: Gene Expression, Western Blot, Expressing, Control